Western blot analysis of extracts of various cell lines, using PD-L1 antibody, catalog # CA011.
Programmed cell death ligand 1 (CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
|Immunogen:||Recombinant protein of human PD-L1|
|Synonyms:||CD274; B7-H; B7H1; MGC142294; MGC142296; PDCD1L1; PDCD1LG1; PDL1|
|Calculated MW:||33 kDa|
|Observed MW:||Refer to figure|
|Recommended dilutions:||WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
|Purification Method:||Affinity purified|
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3|
|Storage Conditions:||Store at -20°C. Avoid freeze / thaw cycles.|