HER2 Antibody (RM228)

Catalog #CA015

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Description

HER2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

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Images


IHC staining of formalin fixed and paraffin embedded human breast cancer tissue sections, using HER2 antibody (clone RM228).


Western Blot analysis of MCF-7, PC3, Jurkat, and A375 cell lysates using HER2 antibody (clone RM228), showed a band of HER2 expressed only in MCF-7 cells.

Product Details

Catalog #: CA015
Clone ID: RM228
Clonality: Monoclonal
Host species: Rabbit
Ig subclass: IgG
Immunogen: A peptide corresponding to the C-terminus of Human c-erbB-2/HER-2
UniProt ID: P04626
Gene ID: 2064
Synonyms: Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, CD340, HER2, NEU, NGL, c-ErbB-2
Observed MW: Refer to figure
Species Reactivity: Human
Specificity: This antibody reacts to Human c-erbB-2/HER-2. It may also react to mouse or rat c-erbB-2/HER-2, as predicted by immunogen homology. No cross reactivity with EGF receptor, HER3 or HER4.
Applications: IHC, WB
Recommended dilutions: IHC: 1:100-1:400
WB: 1:1000
Purification Method: Protein A affinity purified from an animal origin–free culture supernatant
Buffer: 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Storage Conditions: Stable for 1 year at -20.0°C from date of receipt.

100 ul - $249

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