IHC staining of human tonsil tissue with CD8 antibody (catalog at# CA025) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. Multiple transcript variants encoding different isoforms have been found for this gene, such as CD8a, CD8b.This protein is present on virtually all peripheral blood T cells and thymocytes. The CD8 plays an important role in coupling antigen recognition to several intracellular signal transduction pathway.
|Immunogen:||A synthetic peptide corresponding to the N-terminus of human CD8. This sequence is identical among human, rat and mouse species.|
|UniProt ID:||P01732, P10966|
|Gene ID:||925, 926|
|Synonyms:||CD8; MAL; p32; Leu2|
|Specificity:||This antibody recognizes human CD8 protein|
|Applications:||WB, IHC, FC|
|Recommended dilutions:||IHC: 2-5 ug/ml
WB: 0.1-1 ug/ml
FC: 5-10 ug/ml
|Purification Method:||Epitope affinity purified|
|Buffer:||Sterile PBS (pH7.2) containing antibody stabilizer|
|Storage Conditions:||Stable for 1 year at -20.0°C from date of receipt.|