ELISA was performed using coated CD40 antigen (0.1ug per well) and CD40 antibody (CB25), Catalog # C0029.
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. It is also expressed on monocytes, dendritic cells, endothelial cells, and epithelial cells. CD40 plays an essential role in T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation in mice. CD40 interacts with the CD40 ligand, which is found primarily on T cells, playing a role in both humoral and cell-mediated immune responses. Activation of CD40 on B cells by CD40 ligand causes B cell proliferation, differentiation, immunoglobulin isotype switching, germinal center formation, and stimulation of the humoral memory response. CD40 has been found to mediate a broad variety of immune and inflammatory responses. [OMIM: 109535]
This CD40 antibody was developed from mouse immunization, and expressed in suspension culture of mouse hybridoma clone CB25.
|Ig subclass:||Mouse IgG1|
|Immunogen:||Human CD40, ECD domain|
|Synonyms:||B-cell surface antigen CD40, Tumor necrosis factor receptor superfamily member 5, CD40L receptor|
|Recommended dilutions:||ELISA: 0.2-1.0 ug/mL
WB: 1-10 ug/mL
|QC:||Endotoxin level: <2 EU/mg by the LAL method Purity: >95% by SDS-PAGE|
|Production Method:||Expressed from mouse hybridoma clone CB25|
|Purification Method:||Affinity purified|
|Buffer:||PBS, pH 7.4|
|Storage Conditions:||Store at -20°C. Avoid freeze / thaw cycles.
Stable for 12 months from date of purchase when stored as recommended.