ELISA was performed using coated CD276 antigen (0.1ug per well) and CD276 antibody (CB21), Catalog # C0025.
CD276 (B7H3) belongs to the immunoglobulin superfamily, and is thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
This CD276 antibody was developed from mouse immunization, and expressed in suspension culture of mouse hybridoma clone CB21.
|Immunogen:||Human CD276, ECD domain|
|Synonyms:||CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3;|
|Recommended dilutions:||ELISA: 0.2-1.0 ug/mL
FACS: 1-5 ug/mL
|QC:||Endotoxin level: <2 EU/mg by the LAL method Purity: >95% by SDS-PAGE|
|Production Method:||Expressed from mouse hybridoma clone CB21|
|Purification Method:||Affinity purified|
|Buffer:||PBS, pH 7.4|
|Storage Conditions:||Store at -20°C. Avoid freeze / thaw cycles.
Stable for 12 months from date of purchase when stored as recommended.