CD27 Antibody (CB19)

Catalog # C0023

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Description

CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK.

This CD27 antibody was developed from mouse immunization, and expressed in suspension culture of mouse hybridoma clone CB19.

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Images


ELISA was performed using coated CD27 antigen (0.1ug per well) and CD27 antibody (CB19), Catalog # C0023.


FACS analysis of 5ug/mL CD27 antibody (CB19), Catalog # C0023, binding to CD27 cell line (3 x 105 cells per well, Catalog # C2027). Mouse IgG1, kappa isotype control (Catalog # C0005) was used as negative control.


Western blot analysis of 0.1 ug CD27 ECD-hFC recombinant protein and CD27 lysate from CD27 cell line (Catalog # C2027), using 1ug/mL CD27 antibody (CB19), Catalog # C0023.

 

Product Details

Catalog #: C0023
Clone ID: CB19
Host species: Mouse
Ig subclass: IgG2a
Immunogen: Human CD27, ECD domain
UniProt ID: P26842
Gene ID: 939
Synonyms: MGC20393; S152; T14; TNFRSF7; Tp55;
Species Reactivity: Human
Applications: ELISA, FACS
Recommended dilutions: ELISA: 0.2-1.0 ug/mL
FACS: 1-5 ug/mL
WB: 1-10 ug/mL
QC: Endotoxin level: <2 EU/mg by the LAL method Purity: >95% by SDS-PAGE
Production Method: Expressed from mouse hybridoma clone CB19
Purification Method: Affinity purified
Buffer: PBS, pH 7.4
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.
Stable for 12 months from date of purchase when stored as recommended.

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