ELISA was performed using coated CD27 antigen (0.1ug per well) and CD27 antibody (CB18), Catalog # C0022.
CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK.
This CD27 antibody was developed from mouse immunization, and expressed in suspension culture of mouse hybridoma clone CB18.
|Immunogen:||Human CD27, ECD domain|
|Synonyms:||MGC20393; S152; T14; TNFRSF7; Tp55;|
|Applications:||ELISA, FACS, WB|
|Recommended dilutions:||ELISA: 0.2-1.0 ug/mL
FACS: 1-5 ug/mL
|QC:||Endotoxin level: <2 EU/mg by the LAL method Purity: >95% by SDS-PAGE|
|Production Method:||Expressed from mouse hybridoma clone CB18|
|Purification Method:||Affinity purified|
|Buffer:||PBS, pH 7.4|
|Storage Conditions:||Store at -20°C. Avoid freeze / thaw cycles.
Stable for 12 months from date of purchase when stored as recommended.