IHC staining of human tonsil tissue stained with CD25 antibody, (catalog # CA024) at 1:100 for 10 min @ RT. Staining of formalin fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
The IL2 receptor (IL2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220 low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T cell development in fetal and adult thymus. CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation.
|Immunogen:||Recombinant full length (1-272 aa) of human CD25|
|Synonyms:||IL2RA; CD25; IDDM10; IL2R; TCGFR|
|Observed MW:||Refer to figure|
|Specificity:||This antibody recognizes CD25 protein|
|Recommended dilutions:||IHC: 2-5 ug/ml
WB: 0.1-1 ug/ml
|Purification Method:||Affinity purified|
|Buffer:||Sterile PBS (pH7.2) containing antibody stabilizer|
|Storage Conditions:||Stable for 1 year at -20.0°C from date of receipt.|