IHC staining of Jurkat cells fixed and stained with CD11b antibody (catalog # CA022) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
CD11b (Integrin αMβ2, Mac-1, CD11b/CD18) is a non-covalently linked heterodimer of αM and β2 subunits on the surface of leukocytes. It is expressed on the surface of many leukocytes involved in the innate immune system, including monocytes, granulocytes, macrophages and natural killer cells. CD11b consists of two polypeptide chains with molecular weights 95 and 170kDa. The heterodimeric proteins (designated CD11) share a common beta chain CD18 of 95kDa; The alpha chain component of the leukocyte associated antigen is expressed on granulocytes, monocytes and macrophages. CD11b plays a pivotal role in the adhesion and migration of these cells. This antibody reacts with the 170 kDa (CD11b/CD18 heterodimer).
|Immunogen:||Recombinant protein encoding aa 936-1154 of human CD11b.|
|Synonyms:||Integrin alpha-M, ITGAM, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor|
|Observed MW:||Refer to figure|
|Specificity:||This antibody recognizes CD11b protein|
|Recommended dilutions:||IHC: 2-10 ug/ml
WB: 0.1-1 ug/ml
|Purification Method:||Epitope affinity purified|
|Buffer:||50% Glycerol/PBS with 1% BSA and 0.09% sodium azide|
|Storage Conditions:||Stable for 1 year at -20.0°C from date of receipt.|