Western blot analysis of extracts of various cell lines, using B7-H3 antibody, catalog # CA001.
B7-H3 belongs to the immunoglobulin superfamily, and is thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
|Immunogen:||Recombinant protein of human B7-H3|
|Synonyms:||CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3;|
|Calculated MW:||57 kDa|
|Observed MW:||Refer to figure|
|Species Reactivity:||Human, Mouse, Rat|
|Applications:||WB, IHC, FC|
|Recommended dilutions:||WB: 1:500 - 1:2000
IHC: 1:20 - 1:200
FC: 1:20 - 1:50
|Purification Method:||Affinity purified|
|Buffer:||PBS with 0.02% sodium azide, 50% glycerol, pH7.3|
|Storage Conditions:||Store at -20°C. Avoid freeze / thaw cycles.|