Hybridoma generation: Several steps are involved for generating a mono-specific hybridoma clone, including:

• Antigen conjugation: proteins can be used as a stand-alone antigen, and do not require conjugation. Peptide antigens are normally conjugated to a protein carrier in order to generate efficient immune response. We recommend peptides to be conjugated to KLH for immunization, and to BSA for screening.
• Animal immunization: 5 Balb/c mice will immunized for each antigen
• Detection assay development: antigen-specific detection assay is required before the cloning process. Normally either ELISA or dot-blot assay will be used to detect antibody in the serum, or they can also provide quantitative information on the relative concentration of antibody in the serum of each animal.
• Fusion/Cloning: animals with the highest serum antibody titer will be selected for fusion with myeloma cells. Wells with positive signal will be selected for further expansion and cloning via limiting dilution. Up to two clones will be chosen for antibody production. Additional clones can also be requested.