Protein Expression Services

Protein expression and purification are labor intensive and time consuming. Obtaining sufficient amount of proteins in the desirable purity is often the bottleneck of a research project that requires high quality protein for structural biology, HTS, functional validation  for  drug discovery. CrownBio's experienced team can help you to solve this problem by offering multiple parallel expression screening studies to optimize the expression of difficult proteins in a variety of protein expression systems, on both small and large scales. Our comprehensive service spans vector construction through to purification of ready-to-use protein and can be tailored to meet your exact requirements for protein expression services.

Protein Expression Services include:

• Yeast (Pichia pastoris)
• E. Coli
• Cell-free systems (wheat germ agglutinin)
• Baculovirus / insect cells (Sf9, Sf21 or HighFive cells)
• Mammalian cells (HEK293 or CHO)

ProteinExp^TM Packages (PDF) Click here for your protein expression introductory package

• Sequence optimization using a bioinformatics approach
• Quick solubility & protein expression screening using cell free system or small-scale scouting and protein enrichment process
• Application of fusion, mutation, co-expression, chimeric constructs to aid in expression of difficult proteins
• Expertise in membrane bound protein expression services; such as GPCRs
• SeMET incorporation available in all protein expression systems
• Seamless transfer to cGMP scale baculovirus and mammalian cell culture production

Protein Purification