| Recombinant expression vector construction: 3-4 weeks Amplification, isolation, or synthesis of gene of interest Constructing mammalian expression vectors
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| Pilot transient expression: 1-2 weeks Transient expression condition optimization Expression level analysis
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| Stable cell line development: 7-10 weeks Stable transfection Clone selection and screening Top lines selection, culture expanding, and expression level monitoring
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| Production condition optimization and protein purification: 6-8 weeks Large scale production optimization Protein purification and process development
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