Custom Monoclonal Services:

Novel immunization strategies, antigen design, and screening technologies are essential for the successful generation of monoclonal hybridoma cells lines. Our immunization protocols take advantage of using multiple strains of mice along with altered immunization routes, combinations of ligand carriers and dosing strategies to produce as diverse of collective monoclonal antibody population as possible. This polyclonal utility is essential because diversity of clones to immortalization is a major limiting factor in traditional monoclonal generation. Antigens must be carefully designed, especially when dealing with peptides, to generate a robust reagent that is able to recognize conformational changes of the native protein in assay conditions. Designed to meet the needs on many downstream applications as your in preclinical research, Crown Bio’s Select It Antibody Services provide hybridoma development at an affordable price.

SELECT IT Antibody Service packages are streamlines and time-sensitive

Free Antigen Review	Please take advantage of our free Antigen Design and Protocol Review. Without obligation, we will set-up a meeting for you with our scientific staff to discuss your project. We will provide you with an estimate of project feasibility, timelines, and costs. To begin your discussion, simply Click our Quick Quote and Share their expertise!
Project Design and Immunization	Each project begins with a Scope of Project meeting between you and our technical staff in an effort to assure the success of your project. Mice are then immunized according to the schedule below and titer is monitored by ELISA until sufficient titer is reached to warrant fusion between mouse spleen cells and an immortal, non-secreting myeloma cell line.
Cloning	Up to three potential positive cell lines from Phase II will be expanded to produce sufficient culture to preserve the parent cell line and to reclone the identified positives by limiting dilution. The expansion culture of the three selected positive cell lines will be preserved in liquid nitrogen. Up to seventeen additional positive cell lines may be cryopreserved shipped to you. Recloning of selected positives will be performed twice to evaluate antibody production stability. Clones must exhibit >95% antigen-specific antibody production to be considered stable
Deliverables	At the end of the project the resultant cell line is delivered to, and is the property of, the client. As a safety measure Crown Bio can hold, store, and maintain daughter cell lines, which are available to the client at any time.