Although metabolic dysfunctions become manifest in various tissues/organs that have complex, interrelated relationship and that involve numerous signaling pathways, certain specific pathways can be interrogated in defined in vitro assay.
Crown Bioscience is developing in vitro assays using stable cell lines, primary cells, and freshly isolated animal tissues, such as:
- Stable cell lines
- Mesangial cells
- INS-1
- CHO reporter cell line
- Primary animal tissue from various species
Islets: Insulin synthesis and secretion
Adipose: Glucose uptake
Liver: Glycogen synthesis, gluconeogenesis, lipid metabolism
Muscle: Glucose uptake, fatty acid oxidation