Analytical Confidence
Protein molecules are large and complex, and demand high quality QA/QC procedures to ensure proper identity, purity and functionality. The characterization package is as important as the protein itself. We employ a comprehensive suite of tools to characterize the proteins we produce, so that we can help advance your program with the highese confidence possible.
List of protein characterization assays
| Assay | Purpose |
|---|---|
| SDS PAGE | purity |
| Bradford assay, UV spectrophotometer and ELISA | yield / concentration |
| Western Blotting or N-terminal sequencing | identity, phosphorylation |
| HCP-ELISA | host cell protein detection |
| Protein A-ELISA | protein A residue detection |
| Endotoxin Test | product quality |
| Gel filtration (SEC-HPLC) | aggregation, fragmentation, homogeneity |
| Binding ELISA | binding activity |
| MALDI-TOF-MS and LC/MS/MS | identity and purity, M.W., post-translational modifications |
| Dynamic light scattering | size distribution (mono- or poly-disperse) |
| Circular Dichroism (CD) spectroscopy | folding, conformational stability |
| Biochemical & Cell based assay | functionality and activity |
| Immunohistochemistry | application |